TB-500 Dosage Calculator
Working out TB-500 measurements by hand involves several steps: dividing the vial amount by the water volume, converting milligrams to millilitres, then converting millilitres to syringe units. A small slip at any stage can throw off the result. This free TB-500 dosage calculator does the math for you, so you can see the concentration and the exact syringe reading for any amount.
Enter your vial size, the volume of bacteriostatic water you added and the amount you want to measure. The calculator returns the volume in millilitres and the matching units on a standard U-100 insulin syringe. It is built for researchers and is intended for research and educational purposes only.
1 mL = 100 units on a U-100 syringe · 1 mg = 1000 mcg. For research and educational purposes only. Not medical advice.
TB-500 dosage chart
The chart below shows common calculations for a 5 mg vial of TB-500 reconstituted with 2 mL of bacteriostatic water, which gives a concentration of 2.5 mg/mL. Unit values assume a U-100 syringe, where 100 units equals 1 mL.
| Amount | Volume (mL) | Syringe units (U-100) |
|---|---|---|
| 1 mg | 0.40 mL | 40 units |
| 2 mg | 0.80 mL | 80 units |
Changing the water volume changes every number in the chart. With 1 mL of water, the concentration doubles to 5 mg/mL, so 1 mg becomes 0.20 mL (20 units) and 2 mg becomes 0.40 mL (40 units). With 3 mL of water, the concentration drops to about 1.67 mg/mL, so 1 mg becomes 0.60 mL (60 units) and 2 mg becomes 1.20 mL (120 units), which is more than a standard 1 mL syringe holds. This is why the water volume you choose at reconstitution matters, and why the calculator asks for it first.
How to use this calculator
Step 1: Enter your vial size:
Type in the total amount of TB-500 in your vial, in milligrams. This is printed on the vial label, and common sizes are 2 mg, 5 mg and 10 mg. Make sure you enter the figure in the correct unit, since mixing up milligrams and micrograms is one of the most common sources of error. If you are unsure, check the supplier's documentation before continuing.
Step 2: Enter the water volume:
Enter the volume of bacteriostatic water you added, or plan to add, in millilitres. The calculator uses this to work out the concentration by dividing the vial amount by the water volume. If you have not reconstituted yet, try a few different volumes to see which gives syringe readings that are easy to measure accurately. Once you have reconstituted, record the volume on the vial label so you always enter the right figure.
Step 3: Enter the amount you want to measure:
Type in the target amount in milligrams. The calculator handles the conversion, so you do not need to work out the volume yourself. If you are comparing several amounts, run each one separately and note the results, or refer to the chart above for the standard 5 mg and 2 mL setup.
Step 4: Read the results:
The calculator shows the concentration in mg/mL, the volume to draw in millilitres, and the equivalent units on a U-100 syringe. Double-check that the unit reading fits within your syringe capacity. If the result exceeds the syringe's maximum, consider a smaller water volume at reconstitution, which raises the concentration and reduces the volume needed.
Typical TB-500 amounts used in research
TB-500 is a synthetic peptide based on a fragment of thymosin beta-4, a naturally occurring protein studied for its role in cell migration and tissue repair. Most published research on thymosin beta-4 and related fragments has been carried out in cell cultures and animal models. There are no completed, peer-reviewed human trials establishing a dosing protocol for TB-500 specifically, so any figures you see online should be read with that in mind.
Amounts that circulate in community and supplier literature typically describe a loading phase of around 2 to 2.5 mg, twice weekly, followed by lower maintenance amounts given less often. These numbers are anecdotal and are not derived from controlled clinical studies. TB-500 is not approved for human use by the FDA or any other major regulatory agency, and it is on the World Anti-Doping Agency prohibited list. The figures here are summarised for research and educational context only and are not a recommendation.
Frequently asked questions
How many units is 2 mg of TB-500?
It depends on the concentration. With a 5 mg vial and 2 mL of bacteriostatic water (2.5 mg/mL), 2 mg equals 0.80 mL, or 80 units on a U-100 syringe. With 1 mL of water, 2 mg equals 40 units. Enter your own vial size and water volume in the calculator for an exact figure.
How long does a 5 mg vial of TB-500 last?
A 5 mg vial contains 2.5 measurements of 2 mg, or 5 measurements of 1 mg, regardless of how much water is added. Water only changes the volume of each measurement, not the total amount of peptide. Keep in mind that a reconstituted vial also has a limited usable period in the fridge, commonly cited as up to about 28 days with bacteriostatic water.
How often is TB-500 used in research protocols?
Community-reported protocols commonly describe twice-weekly amounts during an initial phase, then less frequent amounts afterward. Animal studies use a wide range of schedules depending on the model and the question being studied. There is no established human schedule, because TB-500 has not been approved or clinically validated for human use.
How do I store reconstituted TB-500?
Keep reconstituted TB-500 in the refrigerator at 2 to 8°C, protected from light, and avoid repeated freezing and thawing. Label the vial with the concentration and the reconstitution date. Discard the solution if it becomes cloudy, develops particles or changes color.
Disclaimer: This calculator and all content on this page are provided for research and educational purposes only and do not constitute medical advice. TB-500 is not approved for human use. Always follow the safety rules and regulations that apply to your research setting.